MagPrep® Silica Particles for Nucleic Acid Purification
The classical methods of isolating nucleic acids (DNA and RNA) rely on density gradient centrifugation and phenol-chloroform extraction.
These two procedures are lengthy, laborious, nearly impossible to automate and they use hazardous chemicals. Today, companies focus on increasing the throughput of nucleic acids purification by using safer procedures.
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Our MagPrep® Silica Particles have been developed to address these needs. For nucleic acids purification, a number of scientists have already replaced the centrifugation and organic extraction steps with our MagPrep® Silica Particles. The silica magnetic beads are positively charged and therefore easily bind the negatively charged nucleic acids.
This system enables laboratories to automatically process tens of thousands of samples per day.
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For further information: fabrice.sultan@merck.fr
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Physical properties of MagPrep® Silica Particles
• Diameter: ~ 1 µm
• Composition: > 95% magnetite
• Surface area: 16 - 22 m2 per mg (non porous)
• Binding capacity: > 10 µg DNA or RNA per mg
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Presentation
• Particle content: 5 % (50 mg/ml).
• Pack sizes: 1 ml, 5 ml and 10 ml. Large volume are available on simple request.
• Buffer: our particles are suspended in water.
• Storage: our products must be stored at 4-8°C.
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Exclusive features of MagPrep® Silica Particles
• Adsorb leucocytes from whole blood
• Adsorb bacteria from growth medium
• Adsorb viruses from plasma
• Bind nucleic acids under chaotropic and under acidic conditions
• Allow washing buffers without ethanol
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Applications of MagPrep® Silica Particles
• Adsorption of bacteria
• Adsorption of viruses
• Adsorption of leucocytes
• Isolation of genomic DNA from blood
• Isolation of genomic DNA from bacteria
• Isolation of viral DNA/RNA
• Purification of PCR fragments
• Dye removal from sequencing reactions
• Isolation of plasmid DNA
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