Fluorescent Microspheres

 


Catalog

Photostable fluorescent microspheres have been developed and patented by our department. These polystyrene microspheres are monodisperse and uniform, ranging from 0.1µm to 4 µm and are also available as plain or functionalized microspheres. These fluorescent microspheres contain a variety of dyes, spanning the visible spectrum from UV (Excitation wavelength = 375nm/ Emission wavelength = 435nm) to red (Excitation wavelength. = 420nm / Emission wavelength = 660nm)

Our Fluorescent Microspheres are supplied at 1% concentration in water (100mg/10ml).

 



Underlined wavelengths represent predominant excitation and emission wavelengths.
X is excitable at the 442nm He-Cd laser line.
XC and Y are well suited to the 488nm and 515nm bands of the Argon laser.


Fluorescent Standard Microspheres for Flow Cytometer
New type of Fluorescent and Monodisperse Microspheres have been developed and patented.Kits,containing those new products, are designed for the routine calibration and control for flow cytometry.
The fluorescent particles have a long term photo-stability and are supplied as ready-to-use reagents.

ESTAFOCUS
Kit for aligning and focusing flow cytometers

• Size of particles : 2 – 2.5 µm
• Argon-ion laser excitation : 488 nm
• Emission range : 500 – 660 nm
• CV fluorescence : < 2 %

 


 

ESTACAL
An intensity calibration kit (different beads suspensions of various % relative fluorescent intensities) recommended for calibrating.

• Size of particles : 2 – 2.5 µm
• Argon-ion laser excitation : 488 nm
• Emission range : 500 – 660 nm
• CV fluorescence : < 4 %


ESTACOLOR
Kit for aligning flow cytometers at different wavelengths.

 


l   Excitation (nm)

 

l  Emission (nm)

360

 

450

488, 514

 

585 - 610

360, 488, 514

 

560 - 670

 




Dye

l Excitation Wavelength (nm)

l Emission (nm)

Color

U

Scintillator or eventually photoescitable at 375

 

White

X

440, 455, 468

480, 510

Yellow

XC

470, 490

525, 560

Pink

Y

515, 555

568, 610

Red

Z

420, 515, 550

656, 720

Red



 

Plain Polystyrene Fluorescent Microspheres

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Product#

Diameter (µm)

Polymer

Catalog No

F-U010

0,076-0,125

Polystyrene

80 380 066

F-U030

0,276-0,325

Polystyrene

39 410 088

F-U050

0,476-0,575

Polystyrene

80 380 067

F-U100

0,900-1,100

Polystyrene

80 380 068

F-U200

1,800-2,200

Polystyrene

39 557 001

F-U300

2,700-3,300

Polystyrene

80 380 069

F-X010

0,076-0,125

Polystyrene

39 373 080

F-X050

0,476-0,575

Polystyrene

39 555 001

F-X100

0,900-1,100

Polystyrene

80 380 070

F-X200

1,800-2,200

Polystyrene

80 380 071

F-X300

2,700-3,300

Polystyrene

80 380 072

F-XC010

0,076-0,125

Polystyrene

39 558 001

F-XC030

0,276-0,325

Polystyrene

39 377 083

F-XC050

0,476-0,575

Polystyrene

80 380 073

F-XC100

0,900-1,100

Polystyrene

80 380 009

F-XC200

1,800-2,200

Polystyrene

39 560 001

F-XC300

2,700-3,300

Polystyrene

39 561 001

F-Y010

0,076-0,125

Polystyrene

80 380 074

F-Y030

0,276-0,325

Polystyrene

39 379 080

F-Y200

1,800-2,200

Polystyrene

39 562 001

F-Y300

2,700-3,300

Polystyrene

39 563 001

F-Z010

0,076-0,125

Polystyrene

80 380 075

F-Z030

0,276-0,325

Polystyrene

80 380 076

F-Z050

0,476-0,575

Polystyrene

80 380 077

F-Z100

0,900-1,100

Polystyrene

80 380 078

F-Z 200

1,800-2,200

Polystyrene

39 564 001

F-Z 300

2,700-3,300

Polystyrene

39 565 001



 

COOH-Modified Fluorescent Microspheres

Product#

Diameter (µm)

Polymer

Catalog No

F1-U010

0,130-0,190

Polystyrene

39 396 088

F1-U030

0,270-0,330

Polystyrene

39 371 083

F1-U050

0,450-0,530

Polystyrene

80 380 079

F1-U080 

0,760-0,940

Polystyrene

39 411 082

F1-U100

0,950-1,100

Polystyrene

80 380 080

F1-X010

0,130-0,190

Polystyrene

39 374 083

F1-X030

0,270-0,330

Polystyrene

39 412 085

F1-X050

0,450-0,530

Polystyrene

39 566 001

F1-X080

0,760-0,940

Polystyrene

39 413 088

F1-X100 

0,950-1,100

Polystyrene

80 380 081

F1-XC010 

0,130-0,190

Polystyrene

80 380 082

F1-XC030

0,270-0,330

Polystyrene

39 414 082

F1-XC050 

0,450-0,530

Polystyrene

39 566 001

F1-XC080

0,760-0,940

Polystyrene

39 415 085

F1-XC100 

0,950-1,100

Polystyrene

80 380 029

F1-Y010

0,130-0,190

Polystyrene

39 378 086

F1-Y030

0,270-0,330

Polystyrene

39 416 088

F1-Y050

0,450-0,530

Polystyrene

39 566 001

F1-Y080

0,760-0,940

Polystyrene

80 380 083

F1-Y100

0,950-1,100

Polystyrene

80 380 084

F1-Z010

0,130-0,190

Polystyrene

39 419 088

F1-Z030

0,270-0,330

Polystyrene

39 420 083

F1-Z050

0,450-0,530

Polystyrene

80 380 085

F1-Z080

0,760-0,940

Polystyrene

39 567 001

F1-Z100

0,950-1,100

Polystyrene

80 380 029




ESTACOLOR
Kit for aligning flow cytometers at different wavelengths

l Excitation (nm)

l Emission (nm)

360

450

488, 514

585 - 610

360, 488, 514

560 - 670


 

Nomenclature system


 


Last update © Copyright Merck-Chimie S.A.S./ Estapor Microspheres, Fontenay Sous Bois, France