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Magnetic particles are the established alternative to conventional chromatography resins in automated high-throughput protocols replacing centrifugation and filtration by simple magnetic separation steps.
The first generation of MagPrep® particles combine this advantage with unique features like high magnetite content and unporous crystal-like surface which allows them to migrate very fast in magnetic fields while binding target molecules without unspecific adsorption.
Our MagPrep® Silica binds RNA as well as DNA with very high efficiency in the presence of chaotropic reagents as well as under mild acidic buffer conditions.
MagPrep® Silica have a particle size of 100-200nm. Homogenized suspensions of the particles are stable for up to 5 minutes without further agitation and magnetic separation takes less than 15 seconds. These properties are particular useful for high-throughput applications. MagPrep® Silica particles meet the most important requirements to solid supports being used in nucleic acid purification protocols.
Three variations of the particles are available and this set allows for selection of the best candidate for the each and every application and sample type in nucleic acid purification procedures.
MagPrep® TiO2 particles (ref. 1.01642) are magnetite crystals coated with a dense layer of titanium dioxide and a mean diameter of 200 nm.
TiO2 is generally regarded as an efficient and robust material for phosphopeptide enrichment. Our new MagPrep® TiO2 is optimised for the enrichment of phosphopeptidfes as well as glycopeptides prior to mass spectrometry. TiO2 magnetic particles can be separated using a magnet or a centrifuge, and then can be used for automated protein phosphorylation studies on complex biological samples.
The second generation of MagPrep® particles are based on a novel production procedure and are truly monodispersed nanoparticles which can be manufactured in the range of 10 to 100 nm with a surface area of 20 - 200 m2/gram. The product line described below represents the first introduction of such novel particles characterized by a magnetite content of over 90% (fast magnetic separation, figure 2) and a particle size of 25nm. To further extend the binding capacity of these particles they are encapsulated with a carboxylated (COOH) tentacle polymer for the covalent immobilization of affinity ligands like protein A, streptavidin or specific antibodies/proteins.
MagPrep® P-25 Protein A (ref. 1.02540) is a suspension of 25 nm particles with Protein A covalently coupled on their surface. Particles are used for capturing immunoglobulins from a variety of samples like plasma, cell culture supernatants or other biological sources. The loaded particles with Protein A-IgG complex can be used for immunoprecipitation of target antigens. In comparison to some competitors products, these new nanoparticles demonstrate a extremely high binding capacity.
The Figure1 give the μg amounts of IgG per mg particle:
MagPrep® P-25 Streptavidin (ref. 1.02550) is a suspension of 25 nm particles covalently coated with Streptavidin. Particles can be used for affinity capture of biotinylated molecules such as proteins and nucleic acids labelled with biotin.
MagPrep® P-25 Carboxy (ref. 1.02510) is a suspension of 25 nm particles coated with a polymer and active carboxy groups which can be used for covalent attachment of ligands to the surface. The amount of protein which can be immobilized on the surface is in the range of 100 μg/mg particles. One of various immobilisation protocols which can be used with this product is available on request.
Figure 2: Magnetophoresis of MagPrep P-25 Protein A measured with SEPMAGR LAB 1x25 ml (data normalized)
